RNA interference mediating small RNA molecules

专利名称：RNA interference mediating small RNA

molecules

发明人：Thomas Tuschl,Sayda Mahgoub

Elbashir,Winfried Lendeckel

申请号：US12897374申请日：20101004公开号：US08895718B2公开日：20141125

摘要：Double-stranded RNA (dsRNA) induces sequence-specific post-transcriptionalgene silencing in many organisms by a process known as RNA interference (RNAi). Using ain vitro system, we demonstrate that 19-23 nt short RNA fragments are the sequence-specific mediators of RNAi. The short interfering RNAs (siRNAs) are generated by anRNase III-like processing reaction from long dsRNA. Chemically synthesized siRNAduplexes with overhanging 3′ ends mediate efficient target RNA cleavage in the lysate,and the cleavage site is located near the center of the region spanned by the guidingsiRNA. Furthermore, we provide evidence that the direction of dsRNA processingdetermines whether sense or antisense target RNA can be cleaved by the producedsiRNP complex.

申请人：Thomas Tuschl,Sayda Mahgoub Elbashir,Winfried Lendeckel

地址：Brooklyn NY US,Cambridge MA US,Hohengandern DE

国籍：US,US,DE

代理机构：Lando & Anastasi, LLP

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